{"id":421,"date":"2022-06-21T14:30:03","date_gmt":"2022-06-21T12:30:03","guid":{"rendered":"https:\/\/research.dii.unipd.it\/hymat\/?page_id=421"},"modified":"2025-08-07T11:50:37","modified_gmt":"2025-08-07T09:50:37","slug":"researc","status":"publish","type":"page","link":"https:\/\/research.dii.unipd.it\/hymat\/researc\/","title":{"rendered":"Research"},"content":{"rendered":"
\r\n
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\r\n
\r\n\r\n\"organoids\r\n

Pancreatic organoids: acinar-to-ductal metaplasia induced by fine tuning of stiffness in designed hyaluronic based hydrogels mimicking ECM<\/p>\r\n\"Breast\r\n

Breast tumor organoids growth within Matrigel, chemically modified cellulose and hyaluronic acid gel<\/p>\r\n\r\n<\/div>\r\n

\r\n

Engineering\r\nthe 3D cell\r\nmicroenvironment<\/span><\/h2>\r\n

Hydrogels are highly swellable 3D networks composed of hydrophilic crosslinked polymer chains, that are widely used as matrices for cell growth due to the similarity of their mechanical and diffusivity properties to the extracellular matrix. We develop hydrogels\u00a0in standardized conditions for organoid culture in synthetic matrices alternative to Matrigel.<\/p>\r\n\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n\r\n

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\r\n
\r\n
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2D hydrogels for mechanobiology<\/strong><\/h2>\r\n

Mechanical signals are pivotal ingredients in how cells perceive and respond to their microenvironments, and to synthetic biomaterials that mimic them. We develop 2D hydrogels substrates recapitulating the physical cues that natural extracellular matrices (ECM) deliver to cells. Cell mechanosignaling and nuclear shape are controlled by independently modulating substrate stiffness and adhesiveness, in static or dynamic conditions.<\/p>\r\n\r\n<\/div>\r\n

\r\n\r\n\"WI68\r\n

1 hour movie of cell cytoskeleton modification during substrate softening.<\/p>\r\n\r\n<\/div>\r\n<\/div>\r\n

<\/div>\r\n
\r\n
\"nuclear<\/div>\r\n
\"stiffness<\/div>\r\n
\"nuclear<\/div>\r\n
\r\n
\r\n

Nuclear cell’s shape on soft PEG hydrogels.<\/p>\r\n\r\n<\/div>\r\n

\r\n

Immunofluorescence showing nuclei, activation state of YAP\/TAZ and F-actin structure at different stiffnesses on designed PEG hydrogels.<\/p>\r\n\r\n<\/div>\r\n

\r\n

Nuclear cell’s shape on stiff PEG hydrogels.<\/p>\r\n\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n

<\/div>\r\n\r\n
\r\n\r\n
\r\n
\r\n
\r\n
\r\n\r\n\"cell-adhesive\r\n

Microfabricated adhesive \u2018islands\u2019 coated with fluorescent fibronectin. Single cell (Human Epidermal Keratinocytes) seeded and shaped by the adhesive area<\/p>\r\n\"MSC\r\n

Cell spreading on big adhesive areas increases YAP\/TAZ nuclear accumulation, whereas confinement leads to YAP\/TAZ turn off. MSCs forced to small pattern (right) differentiate into adipocytes.<\/p>\r\n\"\"\r\n

Single cell (Human Epidermal Keratinocytes) seeded on small round adhesive islands<\/p>\r\n\r\n<\/div>\r\n

\r\n

Engineering\r\ncell size<\/span><\/h2>\r\n

Micropatterned adhesive \u2018islands\u2019 coated with ECM molecules allow restriction of the adhesive area and shape to which individual cells can attach in two dimensions, offering the opportunity to study the relation between cell shape and function. On small islands, cell spreading is reduced, narrowing the distance between focal adhesions, as such inducing a more relaxed cytoskeletal state typified by thicker cortical actin and reduced stress fibres.<\/p>\r\n\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n\r\n

\r\n\r\n
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\r\n

3D\r\nmicrofabrication<\/h2>\r\n

Functional materials are engineered for 3D fabrication of complex structures at the micro, meso and macroscale, by two photon lithography or stereolithography. The interest is common to the scientific community, medical applications and to the industry, for the perspective applications to functional biomimetic interfaces, miniaturized devices, and 3D prototypes.<\/p>\r\n\r\n<\/div>\r\n

\r\n\r\n\"\"\r\n

Microfabricated adhesive \u2018islands\u2019 coated with fluorescent fibronectin. Single cell (Human Epidermal Keratinocytes) seeded and shaped by the adhesive area<\/p>\r\n\"\"\r\n

Cell spreading on big adhesive areas increases YAP\/TAZ nuclear accumulation, whereas confinement leads to YAP\/TAZ turn off. MSCs forced to small pattern (right) differentiate into adipocytes.<\/p>\r\n\"\"\r\n

Single cell (Human Epidermal Keratinocytes) seeded on small round adhesive islands<\/p>\r\n\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n\r\n

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\r\n
\r\n
\r\n\r\n\"\"\r\n

Soft, injectable\u00a0hydrogel\u00a0instructs local immune responses by releasing immunomodulatory cues through controlled in-vivo degradation.<\/p>\r\n\"\"\r\n

Development and characterization of nanocellulose-collagen hydrogels, including their physicochemical and mechanical properties. 3D in vitro neuronal cell cultures demonstrate that the developed hydrogels support cell viability and neurite outgrowth over 14 d,<\/span>ays outperforming collagen and Matrigel.<\/span><\/p>\r\n\r\n<\/div>\r\n

\r\n

Smart<\/strong>\r\nHydrogels<\/strong><\/h2>\r\n

Versatile smart hydrogel platforms are developed by combining different biomaterials.\r\nInjectable hydrogel niches aim to modulate immune responses against disease, while tuneable synthetic hydrogels are engineered to mimic the ECM and support 3D cell cultures.<\/p>\r\n\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>","protected":false},"excerpt":{"rendered":"

Pancreatic organoids: acinar-to-ductal metaplasia induced by fine tuning of stiffness in designed hyaluronic based hydrogels mimicking ECM Breast tumor organoids growth within Matrigel, chemically modified cellulose and hyaluronic acid gel Engineering the 3D cell microenvironment Hydrogels are highly swellable 3D networks composed of hydrophilic crosslinked polymer chains, that are widely used as matrices for cell…
Leggi tutto<\/a><\/p>\n","protected":false},"author":93,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"pro\/page-templates\/full-width-page.php","meta":{"footnotes":""},"folder":[],"class_list":["post-421","page","type-page","status-publish","hentry"],"yoast_head":"\nResearch - HyMat<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.dii.unipd.it\/hymat\/researc\/\" \/>\n<meta property=\"og:locale\" content=\"it_IT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Research - HyMat\" \/>\n<meta property=\"og:description\" content=\"Pancreatic organoids: acinar-to-ductal metaplasia induced by fine tuning of stiffness in designed hyaluronic based hydrogels mimicking ECM Breast tumor organoids growth within Matrigel, chemically modified cellulose and hyaluronic acid gel Engineering the 3D cell microenvironment Hydrogels are highly swellable 3D networks composed of hydrophilic crosslinked polymer chains, that are widely used as matrices for cell… Leggi tutto\" \/>\n<meta property=\"og:url\" content=\"https:\/\/research.dii.unipd.it\/hymat\/researc\/\" \/>\n<meta property=\"og:site_name\" content=\"HyMat\" \/>\n<meta property=\"article:modified_time\" content=\"2025-08-07T09:50:37+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/research.dii.unipd.it\/hymat\/wp-content\/uploads\/sites\/50\/2022\/03\/cropped-organoids-Tito-1.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Tempo di lettura stimato\" \/>\n\t<meta name=\"twitter:data1\" content=\"6 minuti\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/research.dii.unipd.it\\\/hymat\\\/researc\\\/\",\"url\":\"https:\\\/\\\/research.dii.unipd.it\\\/hymat\\\/researc\\\/\",\"name\":\"Research - 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