{"id":575,"date":"2023-01-30T12:49:26","date_gmt":"2023-01-30T11:49:26","guid":{"rendered":"https:\/\/research.dii.unipd.it\/biamet\/?page_id=575"},"modified":"2023-01-30T12:49:33","modified_gmt":"2023-01-30T11:49:33","slug":"extracellular-vesicles-in-cancer-research","status":"publish","type":"page","link":"https:\/\/research.dii.unipd.it\/biamet\/?page_id=575","title":{"rendered":"Extracellular vesicles in cancer research"},"content":{"rendered":"<p>Tumor hypoxia stimulates release of extracellular vesicles (EVs) that facilitate short- and long-range intercellular communication and metastatization. Albeit hypoxia and EVs release are known features of Neuroblastoma (NB), a metastasis-prone childhood malignancy of the sympathetic nervous system, whether hypoxic EVs can facilitate NB dissemination is unclear.EVs derived from hypoxic NB cells were more potent than their normoxic counterpart in inducing NB cells migration and colony formation. We also focused on the effects of EVs in metastatic niches formation <em>in vivo<\/em>. Intravascular injection within the zebrafish embryo allows a non-invasive visualization of EVs dispersion, uptake, and interactions with host cells. We have demonstrated that EVs released under hypoxic conditions promote angiogenesis and are more easily internalized by endothelial cells than those purified from normoxic cells. Furthermore, we have proved via microscopy imaging and cell sorting that the group injected with hypoxic EVs had higher numbers of macrophages.\u00a0 We showed that hypoxic EVs induce <em>i.<\/em> angiogenesis <em>in vivo<\/em>, required for tumor and metastasis growth, and <em>ii.<\/em> increased number of macrophages, a major contributor to the tumor microenvironment which produce growth factors and chemokines promoting tumor angiogenesis. Our data demonstrate that hypoxic EVs can modify the behavior of recipient cells in the CHT, as a potential metastatic site, to prepare a \u2018fertile soil\u2019 for cancer cells colonization.<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-567 size-large\" src=\"https:\/\/research.dii.unipd.it\/biamet\/wp-content\/uploads\/sites\/26\/2023\/01\/ImmagineEVS-1024x792.jpg\" alt=\"\" width=\"1024\" height=\"792\" srcset=\"https:\/\/research.dii.unipd.it\/biamet\/wp-content\/uploads\/sites\/26\/2023\/01\/ImmagineEVS-1024x792.jpg 1024w, https:\/\/research.dii.unipd.it\/biamet\/wp-content\/uploads\/sites\/26\/2023\/01\/ImmagineEVS-300x232.jpg 300w, https:\/\/research.dii.unipd.it\/biamet\/wp-content\/uploads\/sites\/26\/2023\/01\/ImmagineEVS-768x594.jpg 768w, https:\/\/research.dii.unipd.it\/biamet\/wp-content\/uploads\/sites\/26\/2023\/01\/ImmagineEVS.jpg 1247w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tumor hypoxia stimulates release of extracellular vesicles (EVs) that facilitate short- and long-range intercellular communication and metastatization. Albeit hypoxia and EVs release are known features of Neuroblastoma (NB), a metastasis-prone childhood malignancy of the sympathetic nervous system, whether hypoxic EVs can facilitate NB dissemination is unclear.EVs derived from hypoxic NB cells were more potent than&hellip; <br \/> <a class=\"read-more\" href=\"https:\/\/research.dii.unipd.it\/biamet\/?page_id=575\">Leggi tutto<\/a><\/p>\n","protected":false},"author":81,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"folder":[],"class_list":["post-575","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Extracellular vesicles in cancer research - BIAMET Laboratory<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.dii.unipd.it\/biamet\/?page_id=575\" \/>\n<meta property=\"og:locale\" content=\"it_IT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Extracellular vesicles in cancer research - BIAMET Laboratory\" \/>\n<meta property=\"og:description\" content=\"Tumor hypoxia stimulates release of extracellular vesicles (EVs) that facilitate short- and long-range intercellular communication and metastatization. Albeit hypoxia and EVs release are known features of Neuroblastoma (NB), a metastasis-prone childhood malignancy of the sympathetic nervous system, whether hypoxic EVs can facilitate NB dissemination is unclear.EVs derived from hypoxic NB cells were more potent than&hellip; Leggi tutto\" \/>\n<meta property=\"og:url\" content=\"https:\/\/research.dii.unipd.it\/biamet\/?page_id=575\" \/>\n<meta property=\"og:site_name\" content=\"BIAMET Laboratory\" \/>\n<meta property=\"article:modified_time\" content=\"2023-01-30T11:49:33+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/research.dii.unipd.it\/biamet\/wp-content\/uploads\/sites\/26\/2023\/01\/ImmagineEVS-1024x792.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Tempo di lettura stimato\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minuti\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/?page_id=575\",\"url\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/?page_id=575\",\"name\":\"Extracellular vesicles in cancer research - BIAMET Laboratory\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/?page_id=575#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/?page_id=575#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/wp-content\\\/uploads\\\/sites\\\/26\\\/2023\\\/01\\\/ImmagineEVS-1024x792.jpg\",\"datePublished\":\"2023-01-30T11:49:26+00:00\",\"dateModified\":\"2023-01-30T11:49:33+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/?page_id=575#breadcrumb\"},\"inLanguage\":\"it-IT\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/?page_id=575\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"it-IT\",\"@id\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/?page_id=575#primaryimage\",\"url\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/wp-content\\\/uploads\\\/sites\\\/26\\\/2023\\\/01\\\/ImmagineEVS.jpg\",\"contentUrl\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/wp-content\\\/uploads\\\/sites\\\/26\\\/2023\\\/01\\\/ImmagineEVS.jpg\",\"width\":1247,\"height\":965},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/?page_id=575#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Extracellular vesicles in cancer research\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/#website\",\"url\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/\",\"name\":\"BIAMET Laboratory\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"it-IT\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/#organization\",\"name\":\"Universit\u00e0 degli studi di Padova\",\"url\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"it-IT\",\"@id\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/wp-content\\\/uploads\\\/sites\\\/26\\\/2022\\\/12\\\/Immagine2.jpg\",\"contentUrl\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/wp-content\\\/uploads\\\/sites\\\/26\\\/2022\\\/12\\\/Immagine2.jpg\",\"width\":1901,\"height\":394,\"caption\":\"Universit\u00e0 degli studi di Padova\"},\"image\":{\"@id\":\"https:\\\/\\\/research.dii.unipd.it\\\/biamet\\\/#\\\/schema\\\/logo\\\/image\\\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Extracellular vesicles in cancer research - BIAMET Laboratory","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/research.dii.unipd.it\/biamet\/?page_id=575","og_locale":"it_IT","og_type":"article","og_title":"Extracellular vesicles in cancer research - BIAMET Laboratory","og_description":"Tumor hypoxia stimulates release of extracellular vesicles (EVs) that facilitate short- and long-range intercellular communication and metastatization. Albeit hypoxia and EVs release are known features of Neuroblastoma (NB), a metastasis-prone childhood malignancy of the sympathetic nervous system, whether hypoxic EVs can facilitate NB dissemination is unclear.EVs derived from hypoxic NB cells were more potent than&hellip; Leggi tutto","og_url":"https:\/\/research.dii.unipd.it\/biamet\/?page_id=575","og_site_name":"BIAMET Laboratory","article_modified_time":"2023-01-30T11:49:33+00:00","og_image":[{"url":"https:\/\/research.dii.unipd.it\/biamet\/wp-content\/uploads\/sites\/26\/2023\/01\/ImmagineEVS-1024x792.jpg","type":"","width":"","height":""}],"twitter_card":"summary_large_image","twitter_misc":{"Tempo di lettura stimato":"2 minuti"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/research.dii.unipd.it\/biamet\/?page_id=575","url":"https:\/\/research.dii.unipd.it\/biamet\/?page_id=575","name":"Extracellular vesicles in cancer research - BIAMET Laboratory","isPartOf":{"@id":"https:\/\/research.dii.unipd.it\/biamet\/#website"},"primaryImageOfPage":{"@id":"https:\/\/research.dii.unipd.it\/biamet\/?page_id=575#primaryimage"},"image":{"@id":"https:\/\/research.dii.unipd.it\/biamet\/?page_id=575#primaryimage"},"thumbnailUrl":"https:\/\/research.dii.unipd.it\/biamet\/wp-content\/uploads\/sites\/26\/2023\/01\/ImmagineEVS-1024x792.jpg","datePublished":"2023-01-30T11:49:26+00:00","dateModified":"2023-01-30T11:49:33+00:00","breadcrumb":{"@id":"https:\/\/research.dii.unipd.it\/biamet\/?page_id=575#breadcrumb"},"inLanguage":"it-IT","potentialAction":[{"@type":"ReadAction","target":["https:\/\/research.dii.unipd.it\/biamet\/?page_id=575"]}]},{"@type":"ImageObject","inLanguage":"it-IT","@id":"https:\/\/research.dii.unipd.it\/biamet\/?page_id=575#primaryimage","url":"https:\/\/research.dii.unipd.it\/biamet\/wp-content\/uploads\/sites\/26\/2023\/01\/ImmagineEVS.jpg","contentUrl":"https:\/\/research.dii.unipd.it\/biamet\/wp-content\/uploads\/sites\/26\/2023\/01\/ImmagineEVS.jpg","width":1247,"height":965},{"@type":"BreadcrumbList","@id":"https:\/\/research.dii.unipd.it\/biamet\/?page_id=575#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/research.dii.unipd.it\/biamet\/"},{"@type":"ListItem","position":2,"name":"Extracellular vesicles in cancer research"}]},{"@type":"WebSite","@id":"https:\/\/research.dii.unipd.it\/biamet\/#website","url":"https:\/\/research.dii.unipd.it\/biamet\/","name":"BIAMET Laboratory","description":"","publisher":{"@id":"https:\/\/research.dii.unipd.it\/biamet\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/research.dii.unipd.it\/biamet\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"it-IT"},{"@type":"Organization","@id":"https:\/\/research.dii.unipd.it\/biamet\/#organization","name":"Universit\u00e0 degli studi di Padova","url":"https:\/\/research.dii.unipd.it\/biamet\/","logo":{"@type":"ImageObject","inLanguage":"it-IT","@id":"https:\/\/research.dii.unipd.it\/biamet\/#\/schema\/logo\/image\/","url":"https:\/\/research.dii.unipd.it\/biamet\/wp-content\/uploads\/sites\/26\/2022\/12\/Immagine2.jpg","contentUrl":"https:\/\/research.dii.unipd.it\/biamet\/wp-content\/uploads\/sites\/26\/2022\/12\/Immagine2.jpg","width":1901,"height":394,"caption":"Universit\u00e0 degli studi di Padova"},"image":{"@id":"https:\/\/research.dii.unipd.it\/biamet\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/research.dii.unipd.it\/biamet\/index.php?rest_route=\/wp\/v2\/pages\/575","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/research.dii.unipd.it\/biamet\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/research.dii.unipd.it\/biamet\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/research.dii.unipd.it\/biamet\/index.php?rest_route=\/wp\/v2\/users\/81"}],"replies":[{"embeddable":true,"href":"https:\/\/research.dii.unipd.it\/biamet\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=575"}],"version-history":[{"count":1,"href":"https:\/\/research.dii.unipd.it\/biamet\/index.php?rest_route=\/wp\/v2\/pages\/575\/revisions"}],"predecessor-version":[{"id":577,"href":"https:\/\/research.dii.unipd.it\/biamet\/index.php?rest_route=\/wp\/v2\/pages\/575\/revisions\/577"}],"wp:attachment":[{"href":"https:\/\/research.dii.unipd.it\/biamet\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=575"}],"wp:term":[{"taxonomy":"folder","embeddable":true,"href":"https:\/\/research.dii.unipd.it\/biamet\/index.php?rest_route=%2Fwp%2Fv2%2Ffolder&post=575"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}